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(A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, <t>anti-CD14</t> and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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(A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, <t>anti-CD14</t> and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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(A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, <t>anti-CD14</t> and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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anti‐human  (Miltenyi Biotec)
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(A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, <t>anti-CD14</t> and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.
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(A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, anti-CD14 and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: Bloodstream-associated Salmonella Typhimurium and Enteritidis iNTS pathovariants hyper-replicate in human macrophages

doi: 10.64898/2026.01.28.702237

Figure Lengend Snippet: (A) Representative phase contrast images of THP-1 cells untreated versus THP-1 cells differentiated with Condition A (PMA 10 ng/mL for 24 h), Condition B (PMA 10 ng/mL for 24 h followed by a 24 h recovery in fresh media), Condition C (PMA 10 ng/mL for 48 h) and Condition D (PMA 50 ng/mL for 48 h). (B) Representative flow cytometric analysis of THP-1 cells and macrophage-induced cells stained using anti-CD11b, anti-CD14 and anti-CD36 antibodies. (C) Median fluorescence intensity (MFI) of CD11b, CD14 and CD36. Data are displayed as mean ± SD from n ≥ 3. Statistical analyses performed using the Brown-Forsythe and Welch ANOVA test and the Dunnet T3 multiple comparison post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Macrophage surface markers expression was assessed via flow cytometry by staining the cells with anti CD11b (FITC, #130-110-552, Miltenyi Biotec), anti CD14 (APC, #130-110-520, Miltenyi Biotec), anti CD36 (APC-Vio770, #130-110-743, Miltenyi Biotec), anti CD86 (PerCP-Vio700, #130-116-267, Miltenyi Biotec), anti HLA-DR (VioGreen, #130-111-795, Miltenyi Biotec), anti CD163 (PE, #130-112-128, Miltenyi Biotec), anti CD206 (VioBlue, #130-127-809, Miltenyi Biotec) according to manufacturer’s instructions.

Techniques: Staining, Fluorescence, Comparison